Hematobiochemical, serological, and molecular detection of Anaplasma marginale in dromedary camels (Camelus dromedarius) in Al-Najaf desert, Iraq

Background and Aim: Anaplasmosis, an underestimated disease transmitted by ticks, is widespread in ruminants, such as the Arabian camel (dromedary camel). This study aimed to examine presence of Anaplasma marginale dromedary camels Al-Hiadyia region Al-Najaf desert, Iraq, using serological molecular tests. Moreover, hematological biochemical changes infected animals were compared with those healthy controls. Materials Methods: The was conducted on 30 260 severe anemia, pale mucus membranes, progressive emaciation investigate antibodies against A. indirect enzyme-linked immunosorbent assay, followed polymerase chain reaction for selected positive samples targeting a specific major surface protein 5 (MSP5). In addition, parameters measured indicate effect blood profile, mineral status, liver kidney functions. Results: Enzyme-linked assay analysis microscopic examination revealed that 115/260 (44.23%) 87 (33.46%) spp., respectively. MSP5 gene, which unique marginale, amplified. results indicated significant decrease total red cells, hemoglobin, packed cell volume increase mean corpuscular camels, but no difference hemoglobin concentration. there white cells count, lymphocytes, erythrocyte sedimentation rate, platelets. levels aspartate aminotransferase, alanine alkaline phosphatase, urea nitrogen, creatinine, iron copper camels. Cholesterol triglyceride showed variations between diseased Conclusion: To best our knowledge, this first demonstrate Iraq. gene valuable diagnostic target identifying marginale. Keywords: parameters, Camel, reaction.